Elucidating critical disease pathways, biomarkers and targets with unprecedented resolution to inform development of new therapeutics and diagnostics.

 

HiF-seq™ platform enables high efficiency single cell sequencing and is particularly well-suited for frozen tissue sections from post-mortem brain tissue which have been challenging for conventional single cell sequencing technologies.

Using this proprietary technology, we are creating the world’s largest repository of data from high-quality, clinically characterized brain tissues of patients with neurodegenerative diseases including Alzheimer’s and Parkinson’s. With this approach, we can study  genomic information for individual cells.

In parallel, we are developing diagnostic assays for known targets in recognized neurodegenerative pathways, as well as novel targets that have to date been challenging to detect and quantitate as biomarkers.

As the repository of data derived from the HiF-seq platform continues to grow, it will be increasingly valuable for guiding development of diagnostic assays and therapeutics .

 
 

HiF-seq 2.2™ Platform

 

Analysis

 

Developing Diagnostic Assays

  • It all begins with an idea. Maybe you want to launch a business. Maybe you want to turn a hobby into something more. Or maybe you have a creative project to share with the world. Whatever it is, the way you tell your story online can make all the difference.

  • It all begins with an idea. Maybe you want to launch a business. Maybe you want to turn a hobby into something more. Or maybe you have a creative project to share with the world. Whatever it is, the way you tell your story online can make all the difference.

 

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Kam, T.-I., X. Mao, H. Park, S.S. Karuppagounder, S.-C. Chou, G.E. Umanah, S. Brahmachari, S.P. Yun, N. Panicker, R. Chen, S.A. Andrabi, C. Qi, G.G. Poirier, O. Pletnikova, J.C. Troncoso, L.M. Bekris, J.B. Leverenz, A.Pantelyat, H.S. Ko, L.S. Rosenthal, T.M. Dawson and V.L. Dawson “Poly (ADP-ribose) Drives Pathologic a-Synuclein Neurodegeneration.” *Both authors contributed equally, Science 362, eaat8407 (2018). DOI: 10.1126/science.aat8407, PMID: 30385548, PMCID: PMC6431793.

 

Brahmachari, S., C. Yuan, S.S. Karuppagounder, P.Ge, D. Kim, A. Liu, H. Jiang, X. Mao, Y. Lee, D.A. Swing, L. Tessarollo, H.S. Ko, V.L. Dawson and T.M. Dawson. “Parkin interacting substrate zinc finger protein 746 is a pathologic mediator in Parkinson’s disease, Brain, 142:2380-2401 (2019) DOI: 10.1093/brain/awz172 PMID: 31237944, PMCID: PMC6658849.

Pirooznia, S.K., C. Yuan, M. Khan, S.S. Karuppagounder, L. Wang, Y. Xiong, S.U. Kang, Y. Lee, V.L. Dawson and T.M. Dawson. “PARIS induced defects in mitochondrial biogenesis drives dopamine neuron loss under conditions of Parkin or PINK1 deficiency.” Molecular Neurodegneration, Mar 5;15(1):17. doi: 10.1186/s13024-020-00363-x. PMID: 32138754